Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus.
نویسندگان
چکیده
Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.
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ورودعنوان ژورنال:
- Infection and immunity
دوره 50 3 شماره
صفحات -
تاریخ انتشار 1985